Nagoya University

Division of Biological Science, Graduate School of Science Japanese Power to overcome environment ALCA
Group of Plant Physiology


  1. Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T. (2018) Identification and characterization of compounds that affect stomatal movements. Plant & Cell Physiology in press. doi:10.1093/pcp/pcy061
  2. Yoshimura M, Sato A, Kuwata K, Inukai Y, Kinoshita T, Itami K, Tsuchiya Y, Hagihara S (2018) Discovery of shoot branching regulator targeting strigolactone receptor DWARF14. ACS Central Science 4, 230-234. doi:10.1021/acscentsci.7b00554
  3. Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S, Torii KU (2018) Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair. Nature Chemical Biology 14, 299–305. doi:10.1038/nchembio.2555
  4. Bowman J.L., Kohchi T., Yamato K.T. et al. Kinoshita T. (58th/113 authors) (2017) Insights into land plant evolution garnered from the Marchantia polymorpha Genome. Cell 171, 287-304. doi:10.1016/j.cell.2017.09.030.
  5. Peterson J, Inoue S, Kelly SM, Sullivan S, Kinoshita T, Christie JM. (2017) Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1. Journal of Biological Chemistry 292, 13843-13852. doi:10.1074/jbc.M117.799643
  6. Inoue S, Kinoshita T. (2017) Blue light regulation of stomatal opening and the plasma membrane H+-ATPase. Plant Physiology 174, 531-538. doi:10.1104/pp.17.00166
  7. Inoue S, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M, Tabata R, Takemiya A, Murata Y, Doi M, Kinoshita T, Shimazaki K. (2017) Brassinosteroid involvement in Arabidopsis thaliana stomatal opening. Plant & Cell Physiology 58, 1048-1058. doi:10.1093/pcp/pcx049
  8. Nakamura A, Tochio N, Fujioka S, Ito S, Kigawa T, Shimada Y, Matsuoka M, Yoshida S, Kinoshita T, Asami T, Seto H, Nakano T. (2017) Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice. PLOS ONE 12, e0174015. doi:10.1371/journal.pone.0174015.
  9. Wang Y, Kinoshita T. (2017) Measurement of stomatal conductance in rice. Bio-protocol Vol 7, Iss 8. doi:10.21769/BioProtoc.2226
  10. Hayashi M, Inoue S, Ueno Y, Kinoshita T. (2017) A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening. Scientific Reports 7, 45586. doi:10.1038/srep45586
  11. Okumura M, Kinoshita T. (2016) Measurement of ATP hydrolytic activity of plasma membrane H+-ATPase from Arabidopsis thaliana leaves. Bio-protocol Vol 6, Iss 23. doi:10.21769/BioProtoc.2044
  12. Inoue S, Takahashi K, Okumura-Noda H, Kinoshita T. (2016) Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase. Plant & Cell Physiology 57, 2194-2201. doi:10.1093/pcp/pcw136
  13. Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano A, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M. (2016) Loss of function at RAE2, a novel EPFL, is required for awnlessness in cultivated Asian rice. Proc. Natl. Acad. Sci. USA 113(32) 8969–8974. doi:10.1073/pnas.1604849113
  14. Aleman F, Yazaki J, Lee M, Takahashi Y, Kim AY, Kinoshita T, Ecker JR, Schroeder JI. (2016) An ABA-increased interaction of the PYL6 ABA receptor with MYC2 transcription factor: A putative link of ABA and JA signaling. Scientific Reports 6, Article number: 28941. doi:10.1038/srep28941
  15. Yamauchi S, Takemiya A, Sakamoto T, Kurata T, Kinoshita T, Shimazaki K. (2016) Plasma membrane H+-ATPase1 (AHA1) plays a major role in Arabidopsis thaliana for stomatal opening in response to blue light. Plant Physiology 171(4):2731-43. doi:10.1104/pp.16.01581
  16. Takahashi Y, Kinoshita T, Matsumoto M, Shimazaki K. (2016) Inhibition of the Arabidopsis bHLH transcription factor by monomerization through abscisic acid-induced phosphorylation. Plant Journal 87(6):559-67. doi:10.1111/tpj.13217
  17. Takahashi K, Kinoshita T. (2016) The regulation of plant cell expansion: Auxin-induced turgor-driven cell elongation. Molecular Cell Biology of the Growth and Differentiation of Plant Cells 156-173. CRC Press. doi:10.1201/b20316
  18. Toda Y, Wang Y, Takahashi A, Kawai Y, Tada Y, Yamaji N, Ma JF, Ashikari M, Kinoshita T. (2016) Oryza sativa H+-ATPase (OSA) is involved in the regulation of dumbbell-shaped guard cells of rice. Plant & Cell Physiology 57:1220-1230. doi:10.1093/pcp/pcw070
  19. Okumura M, Inoue S, Kuwata K, Kinoshita T. (2016) Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation. Plant Physiology 171:580-589. doi:10.1104/pp. 16.00355
  20. Nakamichi N, Takao S, Kudo T, Kiba T, Wang Y, Kinoshita T, Sakakibara H. (2016) Improvement of Arabidopsis biomass and cold-, drought-, and salinity-stress tolerance by modified circadian clock-associated PSEUDO-RESPONSE REGULATORs. Plant & Cell Physiology 57:1085-1097. doi:10.1093/pcp/pcw057
  21. Kamioka M, Takao S, Suzuki T, Taki K, Higashiyama T, Kinoshita T, Nakamichi N. (2016) Direct repression of evening genes by CIRCADIAN CLOCK-ASSOCIATED1 in the Arabidopsis circadian clock. Plant Cell 28: 696-711. doi:org/10.1105/tpc.15.00737
  22. Nagatoshi Y, Mitsuda N, Hayashi M, Inoue S, Okuma E, Kubo A, Murata Y, Seo M, Saji H, Kinoshita T, Ohme-Takagi M. (2016) GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. Proc. Natl. Acad. Sci. USA 113:4218-4223. doi:10.1073/pnas.1513093113
  23. Tsuchiya Y, Yoshimura M, Sato Y, Kuwata K, Toh S, Holbrook-Smith D, Zhang H, McCourt P, Itami K, Kinoshita T, Hagihara S. (2015) Probing strigolactone receptors in Striga hermonthica with fluorescence. Science 349 (6250):864-868. doi:10.1126/science.aab3831
  24. Satake A, Sakurai G, Kinoshita T (2015) Modeling Strategies for Plant Survival, Growth and Reproduction. Plant & Cell Physiology 56 (4): 583-585. doi:10.1093/pcp/pcv041
  25. Kimura Y, Aoki S, Ando E, Kitatsuji A, Watanabe A, Ohnishi M, Takahashi K, Inoue S, Nakamichi N, Tamada Y, Kinoshita T (2015) A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana.Plant & Cell Physiology 56(4):640-9. doi:10.1093/pcp/pcu214
  26. Takahashi Y, Kinoshita T (2014) Stomatal function has an element of hysteresis. New Phytol 205, 455-457. doi:10.1111/nph.13149
  27. Wang Y, Shimazaki K, Kinoshita T (2014) Multiple roles of the plasma membrane H+-ATPase and its regulation.The Enzymes 35: 191-211. doi:10.1016/B978-0-12-801922-1.00008-7
  28. Hayashi K, Kinoshita T (2014) Abscisic acid receptor hole-in-one. Nature Chemical Biology 10, 414-415. doi:10.1038/nchembio.1529
  29. Tomiyama M, Inoue S , Tsuzuki T, Soda M, Morimoto S, Okigaki Y, Ohishi T, Mochizuki N, Takahashi K, Kinoshita T (2014) Mg-chelatase I subunit 1 and Mg-Protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana. Journal of Plant Research 127, 553-563. doi:10.1007/s10265-014-0636-0
  30. Hayashi Y, Takahashi K, Inoue S, Kinoshita T (2014) Abscisic acid suppresses hypocotyl elongation by dephosphorylating plasma membrane H+-ATPase in Arabidopsis thaliana. Plant & Cell Physiology 55, 845-853. doi:10.1093/pcp/pcu028
  31. Wang Y, Noguchi K, Ono N, Inoue S, Terashima I, Kinoshita T (2014) Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. Proc. Natl. Acad. Sci. USA 111, 533-538. doi:10.1073/pnas.1305438111
  32. Kinoshita T (2013) Flow-limiting valve for ABA signalling in stomatal guard cells. New Phytologist 200,943-945 doi:10.1111/nph.12579
  33. Tsuzuki T, Takahashi K, Tomiyama M, Inoue S, Kinoshita T (2013) Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana. Frontiers in Plant Science 4, 440. doi:10.3389/fpls.2013.00440
  34. Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T (2013) TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsis thaliana. Plant Physiology 162(3),1529-1538. doi:10.1104/pp.113.217984
  35. Takahashi Y, Ebisu Y, Kinoshita T, Doi M, Okuma E, Murata Y, Shimazaki K-i (2013) bHLH transcription factors that facilitate K+ uptake during stomatal opening are repressed by abscisic acid through phosphorylation. Science Signaling 6, ra48. doi:10.1126/scisignal.2003760
  36. Ye Y, Adachi Y, Ye W, Hayashi M, Nakamura Y, Kinoshita T, Mori IC, Murata Y (2013) Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata. Plant Physiology 163(2),600-610. doi:10.1104/pp.113.223826
  37. Takahashi K, Hayashi K, Kinoshita T. (2012) Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis thaliana. Plant Physiology159(2),632-641. doi:10.1104/pp.112.196428
  38. Okumura M, Inoue S, Takahashi K, Ishizaki K, Kohchi T, Kinoshita T. (2012) Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha. Plant Physiology 159(2), 826-834. doi:10.1104/pp.112.195537
  39. Okumura M, Takahashi K, Inoue S, Kinoshita T. (2012) Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte. Plant Signaling & Behavior 7(8), 979-982. doi:10.4161/psb.20936
  40. Zeng H, Liu G, Kinoshita T, Zhang R, Zhu Y, Xu G. (2012) Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H+ ATPase in rice roots. Plant and Soil 357(1), 205-214. doi:10.1007/s11104-012-1136-4
  41. Kinoshita T, Ono N, Hayashi Y, Morimoto S, Nakamura S, Soda M, Kato Y, Ohnishi M, Nakano T, Inoue S, Shimazaki K. (2011) FLOWERING LOCUS T regulates stomatal opening. Current Biology 21, 1232-1238. doi:10.1016/j.cub.2011.06.025
  42. Hayashi M, Inoue S, Takahashi K, Kinoshita T. (2011) Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells. Plant & Cell Physiology 52, 1238-1248. doi:10.1093/pcp/pcr072
  43. Tsuzuki T. Takahashi K. Inoue S. Okigaki Y. Tomiyama M. Hossain M.A. Shimazaki K. Murata Y. Kinoshita T. (2011) Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana. Journal of Plant Research 124, 527-538. doi:10.1007/s10265-011-0426-x
  44. Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K (2011) Functional analyses of the activation loop of phototropin2 in Arabidopsis. Plant Physiology 156, 117-128. doi:10.1104/pp.111.175943
  45. Hayashi M. Kinoshita T. (2011) Crosstalk between blue-light- and aba-signaling pathways in stomatal guard cells. Plant Signaling & Behavior 6, 1662-1664. doi:10.4161/psb.6.11.17800
  46. Kinoshita T. Hayashi Y. (2011) New insights into the regulation of stomatal opening by blue light and the plasma membrane H+-ATPase. International Review of Cell and Molecular Biology 289, 89-115. doi:10.1016/B978-0-12-386039-2.00003-1
  47. Hayashi Y, Nakamura S, Takemiya A, Takahashi Y, Shimazaki K, Kinoshita T. (2010) Biochemical characterization of in vitro phosphorylation and dephosphorylation of the plasma membrane H+-ATPase. Plant & Cell Physiology 51, 1186-1196. doi:10.1093/pcp/pcq078
  48. Tominaga M, Harada A, Kinoshita T, Shimazaki K (2010) Biochemical characterization of calcineurin B-like-interacting protein kinase in Vicia guard cells. Plant & Cell Physiology 51, 408-421. doi:10.1093/pcp/pcq006
  49. Inoue S, Kinoshita T, Matsumoto M, Nakayama K, Doi M, Shimazaki K. (2008) Blue light-induced autophoshporylation of phototropin is a primary step for signaling. Proc. Natl. Acad. Sci. USA 105, 5626-5631. doi:10.1073/pnas.0709189105
  50. Inoue S, Kinoshita T, Takemiya A, Doi M, Shimazaki K. (2008) Leaf positioning of Arabidopsis in response to blue light. Molecular Plant 1, 15-26. doi:10.1093/mp/ssm001
  51. Kong, S-G, Kinoshita T, Shimazaki K, Mochizuki N, Suzuki T, Nagatani A. (2007) The C-terminal kinase fragment of Arabidopsis phototropin 2 triggers constitutive phototropin responses. Plant J. 51, 862-873. doi:10.1111/j.1365-313X.2007.03187.x
  52. Takahashi Y, Kinoshita T, Shimazaki K. (2007) Protein phosphorylation and binding of a 14-3-3 protein in Vicia guard cells in response to ABA. Plant & Cell Physiology 48, 1182-1191. doi:10.1093/pcp/pcm093
  53. Shimazaki K, Doi M, Assmann SM, Kinoshita T (2007) Light regulation of stomatal movement. Annual Review of Plant Biology 58, 219-247. doi:10.1146/annurev.arplant.57.032905.105434
  54. Zhang X, Takemiya A, Kinoshita T, Shimazaki K. (2007) Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cell. Plant & Cell Physiology 48, 715-723. doi:10.1093/pcp/pcm039
  55. Takemiya A, Kinoshita T, Asanuma M, Shimazaki K. (2006) Protein phosphatase 1 positively regulates stomatal opening in response to blue light in Vicia faba. Proc. Natl. Acad. Sci. USA 103, 13549-13554. doi:10.1073/pnas.0602503103
  56. Takemiya A, Inoue S, Doi M, Kinoshita T, Shimazaki K. (2005) Phototropins promote plant growth in response to blue light in low light environments. Plant Cell 17, 1120-1127. doi:10.1105/tpc.104.030049
  57. Kinoshita T, Caño-Delgado A, Seto H, Hiranuma S, Fujioka S, Yoshida S, Chory J. (2005) Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 433, 167-171. doi:10.1038/nature03227
  58. Inoue S, Kinoshita T, Shimazaki K. (2005) Possible involvement of phototropins in leaf movement of kidney bean in response to blue light. Plant Physiology 138, 1994-20041. doi:10.1104/pp.105.062026
  59. Emi T, Kinoshita T, Sakamoto K, Mineyuki Y, Shimazaki K. (2005) Isolation of a protein interacting with vfphot1a in guard cells of Vicia faba. Plant Physiology 138, 1615-1626. doi:10.1104/pp.104.052639
  60. Ueno K, Kinoshita T, Inoue S, Emi T, Shimazaki K. (2005) Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light. Plant & Cell Physiology 46, 955-963. doi:10.1093/pcp/pci104
  61. Zhang X, Wang H, Takemiya A, Song C, Kinoshita T, Shimazaki K. (2004) Inhibition of blue light-dependent H+ pumping by abscisic acid through hydrogen peroxide-induced dephosphorylation of the plasma membrane H+-ATPase in guard cell protoplasts. Plant Physiology 136, 4150-4158. doi:10.1104/pp.104.046573
  62. Ng CKY, Kinoshita T, Pandey S, Shimazaki K, Assmann SM. (2004) Abscisic acid induces rapid subnuclear reorganization in guard cells. Plant Physiology 134, 1327-1331. doi:10.1104/pp.103.034728
  63. Doi M, Shigenaga A, Emi T, Kinoshita T, Shimazaki K (2004) A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant. J. Exp. Bot. 55, 517-523. doi:10.1093/jxb/erh044
  64. Kinoshita T, Emi T, Tomonaga M, Sakamoto K, Shigenaga A, Doi M, Shimazaki K. (2003) Blue light- and phosphorylation-dependent binding of a 14-3-3 protein to phototropins in stomtal guard cells of broad bean. Plant Physiology 133, 1453-1463. doi:10.1104/pp.103.029629
  65. Zhao J, Onduka T, Kinoshita J. Honsho M, Kinoshita T, Shimazaki K, Ito A. (2003) Dual subcellular distribution of cytochrome b5 in plant, cauliflower, cells. Journal of Biochemistry 133, 115-121. doi:10.1093/jb/mvg009
  66. Kinoshita T. and Shimazaki K. (2002) Biochemical evidence for the requirement of 14-3-3 protein binding in activation of the guard-cell plasma membrane H+-ATPase by blue light. Plant & Cell Physiology 43,1359-1365. doi:10.1093/pcp/pcf167
  67. Li, J., Kinoshita, T., Pandey, S., Ng, C. K.-Y., Gygi, S.T., Shimazaki, K. and Assmann, A.M. (2002) Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature 418: 793-797. doi:10.1038/nature00936
  68. Kinoshita T., Doi M., Suetsugu N., Kagawa T., Wada M. and Shimazaki K. (2001) phot1 and phot2 mediate blue light regulation of stomatal opening. Nature 414, 656-660. doi:10.1038/414656a
  69. Kinoshita T. and Shimazaki K. (2001) Analysis of the phosphorylation level in guard-cell plasma membrane H+-ATPase in response to fusicoccin. Plant & Cell Physiology 42, 424-432. doi:10.1093/pcp/pce055
  70. Emi T., Kinoshita T. and Shimazaki K. (2001) Specific binding of vf14-3-3a isoform to the plasma membrane H+-ATPase in response to blue light and fusicoccin in guard cells of broad bean. Plant Physiology 125, 1115-1125. doi:10.1104/pp.125.2.1115
  71. Tominaga M., Kinoshita T. and Shimazaki K. (2001) Guard-cell chloroplasts provides ATP required for H+ pumping in the plasma membrane and stomatal opening. Plant & Cell Physiology 42, 795-802. doi:10.1093/pcp/pce101
  72. Kinoshita T. and Shimazaki K. (1999) Blue light activates the plasma membrane H+-ATPase by phosphorylation of the C-terminus in stomatal guard cells. EMBO Journal 18, 5548-5558. doi:10.1093/emboj/18.20.5548
  73. Kinoshita T. and Shimazaki K. (1999) Characterization of cytosolic cyclophilin in guard cells from Vicia faba L. Plant & Cell Physiology 40, 53-59. [Abstract]
  74. Shimazaki K., Goh, C. and Kinoshita T. (1999) Involvement of intracellular Ca2+ in blue light-dependent proton pumping in guard cell protoplasts from Vicia faba. Physiologia Plantarum 105, 554-561. doi:10.1034/j.1399-3054.1999.105322.x
  75. Kinoshita T. and Shimazaki K. (1997) Involvement of calyculin A- and okadaic acid-sensitive protein phosphatase in the blue light response of stomatal guard cells. Plant & Cell Physiology 38,1281-1285. [Abstract]
  76. Goh C-H., Kinoshita T., Oku T. and Shimazaki K. (1996) Inhibition of blue-light-dependent H+ pumping by abscisic acid in Vicia guard-cell protoplasts. Plant Physiology 111, 433-440. doi:10.1104/pp.111.2.433
  77. Kinoshita T., Nishimura M. and Shimazaki K. (1995) Cytosolic concentration of Ca2+ regulates the plasma membrane H+-ATPase in guard cells of Fava bean. Plant Cell 7, 1333-1342. doi:10.2307/3870106
  78. Kinoshita T. and Shimazaki K. (1995) Evidence for Ca2+-dependent phosphorylation in vitro in guard cells from Vicia faba L. Plant Science 110, 173-180. doi:10.1016/0168-9452(95)04200-E
  79. Kinoshita T., Shimazaki K. and Nishimura M. (1993) Phosphorylation and dephosphorylation of guard-cell proteins from Vicia faba L. in response to light and dark. Plant Physiology 102, 917-923. [Abstract]
  80. Shimazaki, K., Omasa, K., Kinoshita, T. and Nishimura, M. (1993) Properties of the signal transduction pathway in the blue light response of stomatal guard cells of Vicia faba and Commelina benghalensis. Plant & Cell Physiology 34, 1321-1327. [Abstract]
  81. Masamoto, K. Kinoshita, T. Shimazaki, K. (1993) Light-induced de-epoxidation of violaxanthin in guard cell protoplasts of Vicia faba. Plant & Cell Physiology 34, 935-938. [Abstract]
  82. Shimazaki K., Kinoshita T. and Nishimura M. (1992) Involvement of calmodulin and calmodulin-dependent myosin light chain kinase in blue light-dependent H+ pumping by guard cell protoplasts from Vicia faba L. Plant Physiology 99, 1416-1421. doi:10.1104/pp.99.4.1416