Nagoya University

Division of Biological Science, Graduate School of Science Japanese Power to overcome environment ALCA
ITbM
Group of Plant Physiology

Publications

    ★：The paper published primarily by our laboratory.

  1. ★ *Ando E, Taki K, Suzuki T, *Kinoshita T. (2024) A novel semi-dominant mutation in BRASINOSTEROID SIGNALING KINASE1 increases stomatal density. Front. Plant Sci. 15: 1377352. https://doi.org/10.3389/fpls.2024.1377352
  2. ★ *Zeng H, Chen H, Zhang M, Ding M, Yan F, *Kinoshita T, *Zhu Y. (2024) Plasma membrane H+-ATPases in mineral nutrition and the potential application in crop improvement. Trends in Plant Science in press.
  3. ★ Hayashi Y, Fukatsu K, Takahashi K, Kinoshita SN, Kato K, Sakakibara T, Kuwata K, *Kinoshita T. (2024) Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening. Nature Communications 15:1194. https://doi.org/10.1038/s41467-024-45248-5
    Press Release：https://www.nagoya-u.ac.jp/researchinfo/result/2024/02/post-629.html
  4. Katagiri S, Kamiyama Y, Yamashita K, Iizumi S, Suzuki R, Aoi Y, Takahashi F, Kasahara H, Kinoshita T, *Umezawa T (2023) Accumulation of phosphorylated SnRK2 substrate 1 promotes drought escape in Arabidopsis. Plant Cell Physiol. in press. https://doi.org/10.1093/pcp/pcad146
  5. ★ Aihara Y, Maeda B, Goto K, Takahashi K, Toh S, Ye W, Toda Y, Uchida M, Asai E, Sato A, Itami K, *Murakami K, *Kinoshita T (2023) Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals. Nature Communications, 14: 2665. https://doi.org/10.1038/s41467-023-38102-7
    Press Release：https://www.nagoya-u.ac.jp/researchinfo/result/2023/05/post-504.html
  6. Takagi M, Hirata R, Aihara Y, Hayashi Y, Mizutani-Aihara M, Ando E, Yoshimura-Kono M, Tomiyama M, Kinoshita T, *Mine A, *Toda Y (2023) Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images. Plant Cell Physiol. 64: 1301-1310. https://doi.org/10.1093/pcp/pcad018
  7. *Negi J, Obata T, Nishimura S, Song B, Yamagaki S, Ono Y, Okabe M, Hoshino N, Fukatsu K, Tabata R, Yamaguchi K, Shigenobu S, Yamada M, Hasebe M, Sawa S, Kinoshita T, Nishida I, Iba K (2023) PECT1, a rate-limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis. Plant J. 115: 563-576. https://doi.org/10.1111/tpj.16245
  8. Xie W, Liu S, Gao H, Wu J, Liu D, Kinoshita T, *Huang CF (2023) A PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice. Plant Physiology 192: 1498-1516. https://doi.org/10.1093/plphys/kiad122
  9. ★ Ding M, Zhu Y,*Kinoshita T (2023) Stomatal properties of Arabidopsis cauline and rice flag leaves and their contributions to seed production and grain yield. J. Exp. Bot. 74: 1957–1973. https://doi.org/10.1093/jxb/erac492
  10. ★ Kinoshita S, Suzuki T, Kiba T, Sakakibara H, *Kinoshita T (2023) Photosynthetic-product-dependent activation of plasma membrane H+-ATPase and nitrate uptake in Arabidopsis leaves. Plant Cell Physiol. 64, 191-203. https://doi.org/10.1093/pcp/pcac157
    Press Release：https://www.nagoya-u.ac.jp/researchinfo/result/2023/01/post-450.html
  11. ★ Ueda A, Aihara Y, Sato S, Kano K, Mishiro-Sato E, Kitano H, Sato A, *Fujimoto KJ, Yanai T, *Amaike K, *Kinoshita T, *Itami K (2023) Discovery of 2,6-dihalopurines as stomata opening inhibitors: Implication of an LRX-mediated H+-ATPase phosphorylation pathway. ACS Chemical Biology 18, 347-355. DOI: 10.1021/acschembio.2c00771
  12. ★ *Raghavendra AS, *Ye W, *Kinoshita T (2023) pH as a signal and secondary messenger in plant cells. Front. Plant Sci. 14:1148689. https://doi.org/10.3389/fpls.2023.1148689
  13. ★ Soda MN, Hayashi Y, Takahashi K, *Kinoshita T (2022) Tryptophan synthase ß subunit 1 affects stomatal phenotypes in Arabidopsis thaliana. Front. Plant Sci. 13:1011360. https://doi.org/10.3389/fpls.2022.1011360
  14. ★ *Ando E, Kollist H, Fukatsu K, *Kinoshita T, Terashima I (2022) Elevated CO2 induces rapid dephosphorylation of plasma membrane H+-ATPase in guard cells. New Phytologist 236: 2061–2074. doi: 10.1111/nph.18472
    Press Release：https://www.s.u-tokyo.ac.jp/ja/press/2022/8083/
  15. *Friml J, Gallei M, Gelová Z, Johnson A, Mazur E, Monzer A, Rodriguez L, Roosjen M, Verstraeten I, Živanović BD, Zou M, Fiedler L, Giannini C, Grones P, Hrtyan M, Kaufmann WA, Kuhn A, Narasimhan M, Randuch M, Rýdza N, Takahashi K, Tan S, Teplova A, Kinoshita T, Weijers D, Rakusová H (2022) ABP1–TMK auxin perception for global phosphorylation and auxin canalization. Nature 609, 575-581. https://doi.org/10.1038/s41586-022-05187-x
  16. Bashir K, Todaka D, Rasheed S, Matsui A, Ahmad Z, Sako K, Utsumi Y, Vu AT, Tanaka M, Takahashi S, Ishida J, Tsuboi Y, Watanabe S, Kanno Y, Ando E, Shin KC, Seito M, Motegi H, Sato M, Li R, Kikuchi S, Fujita M, Kusano M, Kobayashi M, Habu Y, Nagano AJ, Kawaura K, Kikuchi J, Saito K, Hirai MY, Seo M, Shinozaki K, Kinoshita T, *Seki M (2022) Ethanol-mediated novel survival strategy against drought stress in plants. Plant Cell Physiol.63, 1181-1192. https://doi.org/10.1093/pcp/pcac114
  17. Vu AT, Utsumi Y, Utsumi C, Tanaka M, Takahashi S, Todaka D, Kanno Y, Seo M, Ando E, Sako K, Bashir K, Kinoshita T, Pham XH, *Seki M (2022) Ethanol treatment enhances drought stress avoidance in cassava (Manihot esculenta Crantz). Plant Mol. Biol., 110, 269-285. https://doi.org/10.1007/s11103-022-01300-w
  18. ★ *Inoue S, Hayashi M, HuangS, Yokosho K, Gotoh E, Ikematsu S, Okumura M, Suzuki T, Kamura K, *Kinoshita T, *Ma JF (2022) A tonoplast-localized magnesium transporter is crucial for stomatal opening in Arabidopsis under high Mg2+ conditions. New Phytologist 236:864-877 https://doi.org/10.1111/nph.18410
    Press Release：https://www.nagoya-u.ac.jp/researchinfo/result/upload/20220822_sci.pdf
  19. Uehara TN, Nonoyama T, Taki K, Kuwata K, Sato A, Fujimoto KJ, Hirota T, Matsuo H, Maeda AE, Ono A, Takahara TT, Tsutsui H, Suzuki T, Yanai T, Kay SA, Itami K, Kinoshita T, *Yamaguchi J, *Nakamichi N (2022) Phosphorylation of RNA Polymerase II by CDKC;2 maintains the Arabidopsis circadian clock period. Plant Cell Physiol. 63, 450-462. doi: https://doi.org/10.1093/pcp/pcac011
  20. ★ *Kinoshita SN and *Kinoshita T (2022) A win-win scenario for photosynthesis and the plasma membrane H+ pump. Front. Plant Sci. 13:982485. doi:https://doi.org/10.3389/fpls.2022.982485
  21. ★ Akiyama M†, Sugimoto H†, Inoue S, Takahashi Y, Hayashi M, Hayashi Y, Mizutani M, Ogawa T, Kinoshita D, Ando E, Park M, Gray WM, *Kinoshita T (2022) Type 2C protein phosphatase clade D family members dephosphorylate guard cell plasma membrane H+-ATPase. Plant Physiology, Volume 188, Issue 4, April 2022, Pages 2228–2240, doi:https://doi.org/10.1093/plphys/kiab571
  22. Matsumura M, Nomoto M, Itaya T, Aratani Y, Iwamoto M, Matsuura T, Hayashi Y, Mori T, Skelly MJ, Yamamoto YY, Kinoshita T, Mori IC, Suzuki T, Betsuyaku S, Spoel SH, Toyota M, *Tada Y (2022) Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana. Nature Communications, 13. 1216. doi:https://doi.org/10.1038/s41467-022-28813-8
  23. Uehara T, Nonoyama T, Taki K, Kuwata K, Sato A, Fujimoto K, Hirota T, Matsuo H, Maeda A, Ono A, Takahara T, Tsutsui H, Suzuki T, Yanai T, Kay S, Itami K, Kinoshita T, Yamaguchi J, *Nakamichi N (2022) Phosphorylation of RNA polymerase II by CDKC;2 maintains the Arabidopsis circadian clock period. Plant Cell Physiol. 63: 450-462. doi: https://doi.org/10.1093/pcp/pcac011
  24. Toda Y, Perry GJP, Inoue S, Ito E, Kawakami T, Narouz MR, Takahashi K, Aihara Y, Maeda B, Kinoshita T, Itami K, *Murakami K (2022)Identification of stomatal-regulating molecules from de novo arylamine collection through aromatic C-H amination. Scientific Report 12 Article number: 949, doi:https://doi.org/10.1038/s41598-022-04947-z
  25. ★ *Ye W, *Dong J, *Kinoshita T (2022) Editorial: Stomatal biology and beyond. Front. Plant Sci. 13:848811. doi:https://doi.org/10.3389/fpls.2022.848811
  26. ★ Hayashi Y, *Takahashi Y, Fukatsu K, Tada Y, Takahashi K, Kuwata K, Suzuki T, *Kinoshita T (2021) Identification of abscisic acid-dependent phosphorylated basic helix-loop-helix transcription factors in guard cells of Vicia faba by mass spectrometry. Front. Plant Sci.12:735271. doi:10.3389/fpls.2021.735271
  27. ★ Ren Z, Suolang B, Fujiwara T, Yang D, Saijo Y, *Kinoshita T, *Wang Y (2021) Promotion and upregulation of a plasma membrane proton-ATPase strategy: principles and applications. Front. Plant Sci.12:749337. doi:10.3389/fpls.2021.749337
  28. Lin W, Zhou X, Tang W, Takahashi K, Pan X, Dai J, Ren H, Zhu X, Pan S, Zheng H, Gray WM, Xu T, Kinoshita T, Yang Z (2021) TMK-based cell surface auxin signaling activates cell wall acidification in Arabidopsis. Nature 599: 278-282. doi:https://doi.org/10.1038/s41586-021-03976-4
  29. Li L, Verstraeten I, Roosjen M, Takahashi K, Rodriguez L, Merrin J, Chen J, Shabala L, Smet W, Hong Ren H, Vanneste S, Shabala S, De Rybel B, Weijers D, Kinoshita T, Gray WM, Friml J (2021) Cell surface and intracellular auxin signalling for H+ fluxes in root growth. Nature 599: 273-277. doi:https://doi.org/10.1038/s41586-021-04037-6
  30. ★ Toh S, Takata N, Ando E, Toda Y, Wang Y, Hayashi Y, Mitsuda N, Nagano S, Taniguchi T, Kinoshita T (2021) Overexpression of Plasma Membrane H+-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen. Front. Plant Sci. 12: 766037. doi:10.3389/fpls.2021.766037
  31. ★ Ye W, Koya S, Hayashi Y, Jiang H, Oishi T, Kato K, Fukatsu K and Kinoshita T (2021) Identification of Genes Preferentially Expressed in Stomatal Guard Cells of Arabidopsis thaliana and Involvement of the Aluminum-Activated Malate Transporter 6 Vacuolar Malate Channel in Stomatal Opening. Front. Plant Sci. 12:744991. doi:10.3389/fpls.2021.744991
  32. ★ Ding M, Zhang M, Zeng H, Hayashi Y, Zhu Y, *Kinoshita T (2021) Molecular basis of plasma membrane H+-ATPase function and potential application in the agricultural production. Plant Physiology and Biochemistry 168: 10–16. https://doi.org/10.1016/j.plaphy.2021.09.036
  33. ★ Wang T, Ye W, Wang Y, Zhang M, Aihara Y and Kinoshita T (2021) Protease Inhibitor-Dependent Inhibition of Light-Induced Stomatal Opening. Front. Plant Sci. 12:735328. doi: 10.3389/fpls.2021.735328
  34. Toda Y, Tameshige T, Tomiyama M, Kinoshita T, Shimizu KK (2021) An Affordable Image-Analysis Platform to Accelerate Stomatal Phenotyping During Microscopic Observation. Front. Plant Sci. 12:715309. doi: 10.3389/fpls.2021.715309
  35. ★ *Kinoshita T, Toh S, *Torii K.U. (2021) Chemical control of stomatal function and development. Curr. Opin. Plant Biol. , 60, doi: 102010. 10.1016/j.pbi.2021.102010
  36. ★ Zhang M, Wang Y, Chen X, Xu F, Ding M, Ye W, Kawai Y, Toda Y, Hayashi Y, Suzuki T, Zeng H, Xiao L, Xiao X, Xu J, Guo S, Yan F, Shen Q, Xu G, *Kinoshita T, *Zhu Y (2021) Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis. Nature Communications 12, 735. doi:10.1038/s41467-021-20964-4
    Press Release：https://www.itbm.nagoya-u.ac.jp/ja_backup/research/2021/02/post-28.php
  37. Ye W, Munemasa S, Shinya T, Wu W, Ma T, Lu J, Kinoshita T, Kaku H, Shibuya N, Murata Y (2020) Stomatal immunity against fungal invasion comprises not only chitin-induced stomatal closure but also chitosan-induced guard cell death. Proc. Natl. Acad. Sci. USA 117(34), 20932-20942. doi:10.1073/pnas.1922319117
  38. Higo A, Saihara N, Miura F, Higashi Y, Yamada M, Tamaki S, Ito T, Tarutani Y, Sakamoto T, Fujiwara M, Kurata T, Fukao Y, Moritoh S, Terada R, Kinoshita T, Ito T, Kakutani T, Shimamoto K, Tsuji H (2020) DNA methylation is reconfigured at the onset of reproduction in rice shoot apical meristem. Nature Communications 11, 4079. doi:10.1038/s41467-020-17963-2
  39. Inoue S, Kaiserli E, Zhao X, Waksman T, Takemiya A, Okumura M, Takahashi H, Seki M, Shinozaki K, Endo Y, Sawasaki T, Kinoshita T, Zhang X, Christie JM, Shimazaki K (2020) CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana. Plant Journal 104(3), 679-692. doi:10.1111/tpj.14955
  40. Nakamichi N, Kudo T, Makita N, Kiba T, Kinoshita T, Sakakibara H (2020) Flowering time control in rice by introducing Arabidopsis clock-associated PSEUDO-RESPONSE REGULATOR 5. Bioscience, Biotechnology, and Biochemistry 84:5, 970-979. doi:10.1080/09168451.2020.1719822
  41. Toda Y, Okura F, Ito J, Okada S, Kinoshita T, Tsuji H, Saisho D (2020) Training instance segmentation neural network with synthetic datasets for crop seed phenotyping. Communications Biology 3, 173. doi:10.1038/s42003-020-0905-5
  42. Harada A, Okazaki Y, Kinoshita T, Nagai R, Takagi S (2020) Role of proton motive force in photoinduction of cytoplasmic streaming in Vallisneria mesophyll cells. Plants 9(3), 376. doi:10.3390/plants9030376
  43. Ishishita K, Higa T, Tanaka H, Inoue S, Chung A, Ushijima T, Matsushita T, Kinoshita T, Nakai M, Wada M, Suetsugu N, Gotoh E (2020) Phototropin2 Contributes to the Chloroplast Avoidance Response at the Chloroplast-Plasma Membrane Interface. Plant Physiology 183(1), 304-316. doi:10.1104/pp.20.00059
  44. Ye W, Ando E, Rhaman MS, Tahjib-Ul-Arif M, Okuma E, Nakamura Y, Kinoshita T, Murata Y. (2020) Inhibition of light-induced stomatal opening by allyl isothiocyanate does not require guard cell cytosolic Ca2+ signaling. Journal of Experimental Botany 71(10), 2922-2932. doi:10.1101/550491
  45. Busch FA, Tominaga J, Muroya M, Shirakami N, Takahashi S, Yamori W, Kitaoka T, Milward S, Nishimura K, Matsunami E, Toda Y, Higushi C, Muranaka A, Takami T, Watanabe S, Kinoshita T, Sakamoto W, Sakamoto A, Shimada H (2020) Overexpression of BUNDLE SHEATH DEFECTIVE 2 improves the efficiency of photosynthesis and growth in Arabidopsis. Plant Journal 102(1), 129-137. doi: 10.1111/tpj.14617
  46. Hayashi M, Sugimoto H, Takahashi H, Seki M, Shinozaki K, Sawasaki T, Kinoshita T, Inoue S. (2019) Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation in Arabidopsis. Photochem. Photobiol. Sci. 19(1), 88-98. doi:10.1039/C9PP00329K
  47. Kesten C, Gámez-Arjona FM, Menna A, Scholl S, Dora S, Huerta AI, Huang HY, Tintor N, Kinoshita T, Rep M, Krebs M, Schumacher K, Sánchez-Rodríguez C (2019) Pathogen-induced pH changes regulate the growth-defense balance in plants. EMBO Journal 38, e101822. doi:10.1101/550491
  48. Kubo Y, Satoh S, Suzuki H, Kinoshita T, Nakajima N. (2019) Characterization of ethylene-mediated curling of Japanese radish (Raphanus sativus var. longipinnatus) cotyledons. HortScience 54(11), 1896–1901. doi:10.21273/HORTSCI14301-19
  49. Saito AN, Matsuo H, Kuwata K, Ono A, Kinoshita T, Yamaguchi J, Nakamichi N. (2019) Structure–function study of a novel inhibitor of the casein kinase 1 family in Arabidopsis thaliana. Plant Direct 3(9), e00172. doi:10.1002/pld3.172
  50. Ono A, Sato A, Fujimoto KJ, Matsuo H, Yanai T, Kinoshita T, Nakamichi N. (2019) 3, 4-Dibromo-7-Azaindole modulates Arabidopsis circadian clock by inhibiting casein kinase 1 activity. Plant & Cell Physiology 60(11), 2360–2368. doi:10.1093/pcp/pcz183
  51. ★ Aoki S, Toh S, Nakamichi N, Hayashi Y, Wang Y, Suzuki T, Tsuji H, Kinoshita T. (2019) Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana. Scientific Reports 9, 10054. doi:10.1038/s41598-019-46440-0
    Press Release：https://www.itbm.nagoya-u.ac.jp/ja_backup/research/2019/07/post-13.php
  52. Uehara TN, Mizutani Y, Kuwata K, Hirota T, Sato A, Mizoi J, Takao S, Matsuo H, Suzuki T, Ito S, Saito AN, Nishiwaki-Ohkawa T, Yamaguchi-Shinozaki K, Yoshimura T, Kay SA, Itami K, Kinoshita T, Yamaguchi J, Nakamichi N. (2019) Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock. Proc. Natl. Acad. Sci. USA 116(23), 11528-11536. doi:10.1073/pnas.1903357116
  53. Oochi A, Hajny J, Fukui K, Nakao Y, Gallei M, Quareshy M, Takahashi K, Kinoshita T, Harborough SR, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. (2019) Pinstatic acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology 180(2), 1152-1165. doi:10.1104/pp.19.00201
  54. Tanaka-Takada N, Kobayashi A, Takahashi H, Kamiya T, Kinoshita T, Maeshima M. (2019) Plasma membrane-associated Ca2+-binding protein PCaP1 is involved in root hydrotropism of Arabidopsis thaliana. Plant & Cell Physiology 60(6), 1331–1341. doi:10.1093/pcp/pcz042
  55. ★ Minami A, Takahashi K, Inoue S, Tada Y, Kinoshita T. (2019) Brassinosteroid induces phosphorylation of the plasma membrane H+-ATPase during hypocotyl elongation in Arabidopsis thaliana. Plant & Cell Physiology 60(5):935-944. doi:10.1093/pcp/pcz005
  56. Toda Y, Kudo T, Kinoshita T, Nakamichi N. (2019) Evolutionary Insight into the Clock-Associated PRR5 Transcriptional Network of Flowering Plants. Scientific Reports, 9, 2983. doi: 10.1038/s41598-019-39720-2
  57. Asaoka M, Inoue S, Gunji S, Kinoshita T, Maeshima M, Tsukaya H, Ferjani A. (2019) Excess pyrophosphate within guard cells delays stomatal closure. Plant & Cell Physiology 60(4), 875–887. doi:10.1093/pcp/pcz002
  58. ★ Ando E, Kinoshita T. (2019) Fluence rate dependence of red light-induced phosphorylation of plasma membrane H+-ATPase in stomata. Plant Signaling & Behavior 14(2):1561107. doi:10.1080/15592324.2018.1561107
  59. Uraguchi D, Kuwata K, Hijikata Y, Yamaguchi R, Imaizumi H, Sathiyanarayanan AM, Rakers D, Mori N, Akiyama K, Irle S, McCourt P, Kinoshita T, Ooi T, Tsuchiya Y. (2018) A femto-molar range suicide germination stimulant for the parasitic plant Striga hermonthica. Science 362, 1301-1305. doi:10.1126/science.aau5445
  60. ★ Ando E, Kinoshita T. (2018) Red light-induced phosphorylation of plasma membrane H+-ATPase in stomatal guard cells. Plant Physiology 178, 838-849. doi:https://doi.org/10.1104/pp.18.00544
  61. ★ Kinoshita T, McCourt P, Asami T, Torii KU. (2018) Plant chemical biology. Plant & Cell Physiology 59, 1483-1486. doi:10.1093/pcp/pcy142
  62. Yamada R, Murai K, Uchida N, Takahashi K, Iwasaki R, Tada Y, Kinoshita T, Itami K, Torii KU, Hagihara S. (2018) A super strong engineered auxin-TIR1 pair. Plant & Cell Physiology 59, 1538-1544. doi:10.1093/pcp/pcy127
  63. Nishimura N, Tsuchiya W, Moresco JJ, Hayashi Y, Satoh K, Kaiwa N, Irisa T, Kinoshita T, Schroeder JI, Yates III JR, Hirayama T, Yamazaki T. (2018) Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. Nature Communications 9, Article number: 2132. doi:10.1038/s41467-018-04437-9
  64. ★ Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T. (2018) Identification and characterization of compounds that affect stomatal movements. Plant & Cell Physiology 59, 1568-1580. doi:10.1093/pcp/pcy061
    Press Release：https://www.itbm.nagoya-u.ac.jp/ja_backup/research/2018/04/SCL-Kinoshita.php
  65. Yoshimura M, Sato A, Kuwata K, Inukai Y, Kinoshita T, Itami K, Tsuchiya Y, Hagihara S (2018) Discovery of shoot branching regulator targeting strigolactone receptor DWARF14. ACS Central Science 4, 230-234. doi:10.1021/acscentsci.7b00554
  66. Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S, Torii KU (2018) Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair. Nature Chemical Biology 14, 299–305. doi:10.1038/nchembio.2555
  67. Bowman J.L., Kohchi T., Yamato K.T. et al. Kinoshita T. (58th/113 authors) (2017) Insights into land plant evolution garnered from the Marchantia polymorpha Genome. Cell 171, 287-304. doi:10.1016/j.cell.2017.09.030.
  68. Peterson J, Inoue S, Kelly SM, Sullivan S, Kinoshita T, Christie JM. (2017) Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1. Journal of Biological Chemistry 292, 13843-13852. doi:10.1074/jbc.M117.799643
  69. ★ Inoue S, Kinoshita T. (2017) Blue light regulation of stomatal opening and the plasma membrane H+-ATPase. Plant Physiology 174, 531-538. doi:10.1104/pp.17.00166
  70. Inoue S, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M, Tabata R, Takemiya A, Murata Y, Doi M, Kinoshita T, Shimazaki K. (2017) Brassinosteroid involvement in Arabidopsis thaliana stomatal opening. Plant & Cell Physiology 58, 1048-1058. doi:10.1093/pcp/pcx049
  71. Nakamura A, Tochio N, Fujioka S, Ito S, Kigawa T, Shimada Y, Matsuoka M, Yoshida S, Kinoshita T, Asami T, Seto H, Nakano T. (2017) Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice. PLOS ONE 12, e0174015. doi:10.1371/journal.pone.0174015.
  72. ★ Wang Y, Kinoshita T. (2017) Measurement of stomatal conductance in rice. Bio-protocol Vol 7, Iss 8. doi:10.21769/BioProtoc.2226
  73. ★ Hayashi M, Inoue S, Ueno Y, Kinoshita T. (2017) A Raf-like protein kinase BHP mediates blue light-dependent stomatal opening. Scientific Reports 7, 45586. doi:10.1038/srep45586
    Press Release：https://www.itbm.nagoya-u.ac.jp/ja_backup/research/2017/04/BHP-stomata.php
  74. ★ Okumura M, Kinoshita T. (2016) Measurement of ATP hydrolytic activity of plasma membrane H+-ATPase from Arabidopsis thaliana leaves. Bio-protocol Vol 6, Iss 23. doi:10.21769/BioProtoc.2044
  75. ★ Inoue S, Takahashi K, Okumura-Noda H, Kinoshita T. (2016) Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase. Plant & Cell Physiology 57, 2194-2201. doi:10.1093/pcp/pcw136
  76. Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano A, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M. (2016) Loss of function at RAE2, a novel EPFL, is required for awnlessness in cultivated Asian rice. Proc. Natl. Acad. Sci. USA 113(32) 8969–8974. doi:10.1073/pnas.1604849113
  77. Aleman F, Yazaki J, Lee M, Takahashi Y, Kim AY, Kinoshita T, Ecker JR, Schroeder JI. (2016) An ABA-increased interaction of the PYL6 ABA receptor with MYC2 transcription factor: A putative link of ABA and JA signaling. Scientific Reports 6, Article number:28941. doi:10.1038/srep28941
  78. Yamauchi S, Takemiya A, Sakamoto T, Kurata T, Kinoshita T, Shimazaki K. (2016) Plasma membrane H+-ATPase1 (AHA1) plays a major role in Arabidopsis thaliana for stomatal opening in response to blue light. Plant Physiology 171(4):2731-43. doi:10.1104/pp.16.01581
  79. Takahashi Y, Kinoshita T, Matsumoto M, Shimazaki K. (2016) Inhibition of the Arabidopsis bHLH transcription factor by monomerization through abscisic acid-induced phosphorylation. Plant Journal 87(6):559-67. doi:10.1111/tpj.13217
  80. ★ Takahashi K, Kinoshita T. (2016) The regulation of plant cell expansion: Auxin-induced turgor-driven cell elongation. Molecular Cell Biology of the Growth and Differentiation of Plant Cells 156-173. CRC Press. doi:10.1201/b20316
  81. ★ Toda Y, Wang Y, Takahashi A, Kawai Y, Tada Y, Yamaji N, Ma JF, Ashikari M, Kinoshita T. (2016) Oryza sativa H+-ATPase (OSA) is involved in the regulation of dumbbell-shaped guard cells of rice. Plant & Cell Physiology 57:1220-1230. doi:10.1093/pcp/pcw070
  82. ★ Okumura M, Inoue S, Kuwata K, Kinoshita T. (2016) Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation. Plant Physiology 171:580-589. doi:10.1104/pp. 16.00355
  83. Nakamichi N, Takao S, Kudo T, Kiba T, Wang Y, Kinoshita T, Sakakibara H. (2016) Improvement of Arabidopsis biomass and cold-, drought-, and salinity-stress tolerance by modified circadian clock-associated PSEUDO-RESPONSE REGULATORs. Plant & Cell Physiology 57:1085-1097. doi:10.1093/pcp/pcw057
  84. Kamioka M, Takao S, Suzuki T, Taki K, Higashiyama T, Kinoshita T, Nakamichi N. (2016) Direct repression of evening genes by CIRCADIAN CLOCK-ASSOCIATED1 in the Arabidopsis circadian clock. Plant Cell 28: 696-711. doi:org/10.1105/tpc.15.00737
  85. Nagatoshi Y, Mitsuda N, Hayashi M, Inoue S, Okuma E, Kubo A, Murata Y, Seo M, Saji H, Kinoshita T, Ohme-Takagi M. (2016) GOLDEN 2-LIKE transcription factors for chloroplast development affect ozone tolerance through the regulation of stomatal movement. Proc. Natl. Acad. Sci. USA 113:4218-4223. doi:10.1073/pnas.1513093113
  86. Tsuchiya Y, Yoshimura M, Sato Y, Kuwata K, Toh S, Holbrook-Smith D, Zhang H, McCourt P, Itami K, Kinoshita T, Hagihara S. (2015) Probing strigolactone receptors in Striga hermonthica with fluorescence. Science 349 (6250):864-868. doi:10.1126/science.aab3831
  87. ★ Satake A, Sakurai G, Kinoshita T (2015) Modeling Strategies for Plant Survival, Growth and Reproduction. Plant & Cell Physiology 56 (4):583-585. doi:10.1093/pcp/pcv041
  88. ★ Kimura Y, Aoki S, Ando E, Kitatsuji A, Watanabe A, Ohnishi M, Takahashi K, Inoue S, Nakamichi N, Tamada Y, Kinoshita T (2015) A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana.Plant & Cell Physiology 56(4):640-9. doi:10.1093/pcp/pcu214
  89. ★ Takahashi Y, Kinoshita T (2014) Stomatal function has an element of hysteresis. New Phytol 205, 455-457. doi:10.1111/nph.13149
  90. ★ Wang Y, Shimazaki K, Kinoshita T (2014) Multiple roles of the plasma membrane H+-ATPase and its regulation.The Enzymes 35: 191-211. doi:10.1016/B978-0-12-801922-1.00008-7
  91. Hayashi K, Kinoshita T (2014) Abscisic acid receptor hole-in-one. Nature Chemical Biology 10, 414-415. doi:10.1038/nchembio.1529
  92. ★ Tomiyama M, Inoue S , Tsuzuki T, Soda M, Morimoto S, Okigaki Y, Ohishi T, Mochizuki N, Takahashi K, Kinoshita T (2014) Mg-chelatase I subunit 1 and Mg-Protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana. Journal of Plant Research 127, 553-563. doi:10.1007/s10265-014-0636-0
  93. Hayashi Y, Takahashi K, Inoue S, Kinoshita T (2014) Abscisic acid suppresses hypocotyl elongation by dephosphorylating plasma membrane H+-ATPase in Arabidopsis thaliana. Plant & Cell Physiology 55, 845-853. doi:10.1093/pcp/pcu028
  94. ★ Wang Y, Noguchi K, Ono N, Inoue S, Terashima I, Kinoshita T (2014) Overexpression of plasma membrane H+-ATPase in guard cells promotes light-induced stomatal opening and enhances plant growth. Proc. Natl. Acad. Sci. USA 111, 533-538. doi:10.1073/pnas.1305438111
    Press Release：https://www.itbm.nagoya-u.ac.jp/ja_backup/research/2014/01/Kinoshita-Stomata.php
  95. ★ Kinoshita T (2013) Flow-limiting valve for ABA signalling in stomatal guard cells. New Phytologist 200, 943-945. doi:10.1111/nph.12579
  96. ★ Tsuzuki T, Takahashi K, Tomiyama M, Inoue S, Kinoshita T (2013) Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana. Frontiers in Plant Science 4, 440. doi:10.3389/fpls.2013.00440
  97. ★ Ando E, Ohnishi M, Wang Y, Matsushita T, Watanabe A, Hayashi Y, Fujii M, Ma JF, Inoue S, Kinoshita T (2013) TWIN SISTER OF FT, GIGANTEA, and CONSTANS have a positive but indirect effect on blue light-induced stomatal opening in Arabidopsis thaliana. Plant Physiology 162(3),1529-1538. doi:10.1104/pp.113.217984
  98. Takahashi Y, Ebisu Y, Kinoshita T, Doi M, Okuma E, Murata Y, Shimazaki K-i (2013) bHLH transcription factors that facilitate K+ uptake during stomatal opening are repressed by abscisic acid through phosphorylation. Science Signaling 6, ra48. doi:10.1126/scisignal.2003760
  99. Ye Y, Adachi Y, Ye W, Hayashi M, Nakamura Y, Kinoshita T, Mori IC, Murata Y (2013) Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata. Plant Physiology 163(2),600-610. doi:10.1104/pp.113.223826
  100. ★ Takahashi K, Hayashi K, Kinoshita T. (2012) Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis thaliana. Plant Physiology159(2),632-641. doi:10.1104/pp.112.196428
    Press Release
  101. ★ Okumura M, Inoue S, Takahashi K, Ishizaki K, Kohchi T, Kinoshita T. (2012) Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha. Plant Physiology 159(2), 826-834. doi:10.1104/pp.112.195537
  102. ★ Okumura M, Takahashi K, Inoue S, Kinoshita T. (2012) Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte. Plant Signaling & Behavior 7(8), 979-982. doi:10.4161/psb.20936
  103. Zeng H, Liu G, Kinoshita T, Zhang R, Zhu Y, Xu G. (2012) Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H+ ATPase in rice roots. Plant and Soil 357(1), 205-214. doi:10.1007/s11104-012-1136-4
  104. ★ Kinoshita T, Ono N, Hayashi Y, Morimoto S, Nakamura S, Soda M, Kato Y, Ohnishi M, Nakano T, Inoue S, Shimazaki K. (2011) FLOWERING LOCUS T regulates stomatal opening. Current Biology 21, 1232-1238. doi:10.1016/j.cub.2011.06.025
    Press Release
  105. ★ Hayashi M, Inoue S, Takahashi K, Kinoshita T. (2011) Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells. Plant & Cell Physiology 52, 1238-1248. doi:10.1093/pcp/pcr072
  106. ★ Tsuzuki T. Takahashi K. Inoue S. Okigaki Y. Tomiyama M. Hossain M.A. Shimazaki K. Murata Y. Kinoshita T. (2011) Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana. Journal of Plant Research 124, 527-538. doi:10.1007/s10265-011-0426-x
  107. Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K (2011) Functional analyses of the activation loop of phototropin2 in Arabidopsis. Plant Physiology 156, 117-128. doi:10.1104/pp.111.175943
  108. ★ Hayashi M. Kinoshita T. (2011) Crosstalk between blue-light- and aba-signaling pathways in stomatal guard cells. Plant Signaling & Behavior 6, 1662-1664. doi:10.4161/psb.6.11.17800
  109. ★ Kinoshita T. Hayashi Y. (2011) New insights into the regulation of stomatal opening by blue light and the plasma membrane H+-ATPase. International Review of Cell and Molecular Biology 289, 89-115. doi:10.1016/B978-0-12-386039-2.00003-1
  110. ★ Hayashi Y, Nakamura S, Takemiya A, Takahashi Y, Shimazaki K, Kinoshita T. (2010) Biochemical characterization of in vitro phosphorylation and dephosphorylation of the plasma membrane H+-ATPase. Plant & Cell Physiology 51, 1186-1196. doi:10.1093/pcp/pcq078
  111. Tominaga M, Harada A, Kinoshita T, Shimazaki K (2010) Biochemical characterization of calcineurin B-like-interacting protein kinase in Vicia guard cells. Plant & Cell Physiology 51, 408-421. doi:10.1093/pcp/pcq006
  112. Inoue S, Kinoshita T, Matsumoto M, Nakayama K, Doi M, Shimazaki K. (2008) Blue light-induced autophoshporylation of phototropin is a primary step for signaling. Proc. Natl. Acad. Sci. USA 105, 5626-5631. doi:10.1073/pnas.0709189105
  113. Inoue S, Kinoshita T, Takemiya A, Doi M, Shimazaki K. (2008) Leaf positioning of Arabidopsis in response to blue light. Molecular Plant 1, 15-26. doi:10.1093/mp/ssm001
  114. Kong, S-G, Kinoshita T, Shimazaki K, Mochizuki N, Suzuki T, Nagatani A. (2007) The C-terminal kinase fragment of Arabidopsis phototropin 2 triggers constitutive phototropin responses. Plant J. 51, 862-873. doi:10.1111/j.1365-313X.2007.03187.x
  115. Takahashi Y, Kinoshita T, Shimazaki K. (2007) Protein phosphorylation and binding of a 14-3-3 protein in Vicia guard cells in response to ABA. Plant & Cell Physiology 48, 1182-1191. doi:10.1093/pcp/pcm093
  116. Shimazaki K, Doi M, Assmann SM, Kinoshita T (2007) Light regulation of stomatal movement. Annual Review of Plant Biology 58, 219-247. doi:10.1146/annurev.arplant.57.032905.105434
  117. Zhang X, Takemiya A, Kinoshita T, Shimazaki K. (2007) Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cell. Plant & Cell Physiology 48, 715-723. doi:10.1093/pcp/pcm039
  118. Takemiya A, Kinoshita T, Asanuma M, Shimazaki K. (2006) Protein phosphatase 1 positively regulates stomatal opening in response to blue light in Vicia faba. Proc. Natl. Acad. Sci. USA 103, 13549-13554. doi:10.1073/pnas.0602503103
  119. Takemiya A, Inoue S, Doi M, Kinoshita T, Shimazaki K. (2005) Phototropins promote plant growth in response to blue light in low light environments. Plant Cell 17, 1120-1127. doi:10.1105/tpc.104.030049
  120. Kinoshita T, Caño-Delgado A, Seto H, Hiranuma S, Fujioka S, Yoshida S, Chory J. (2005) Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 433, 167-171. doi:10.1038/nature03227
  121. Inoue S, Kinoshita T, Shimazaki K. (2005) Possible involvement of phototropins in leaf movement of kidney bean in response to blue light. Plant Physiology 138, 1994-20041. doi:10.1104/pp.105.062026
  122. Emi T, Kinoshita T, Sakamoto K, Mineyuki Y, Shimazaki K. (2005) Isolation of a protein interacting with vfphot1a in guard cells of Vicia faba. Plant Physiology 138, 1615-1626. doi:10.1104/pp.104.052639
  123. Ueno K, Kinoshita T, Inoue S, Emi T, Shimazaki K. (2005) Biochemical characterization of plasma membrane H+-ATPase activation in guard cell protoplasts of Arabidopsis thaliana in response to blue light. Plant & Cell Physiology 46, 955-963. doi:10.1093/pcp/pci104
  124. Zhang X, Wang H, Takemiya A, Song C, Kinoshita T, Shimazaki K. (2004) Inhibition of blue light-dependent H+ pumping by abscisic acid through hydrogen peroxide-induced dephosphorylation of the plasma membrane H+-ATPase in guard cell protoplasts. Plant Physiology 136, 4150-4158. doi:10.1104/pp.104.046573
  125. Ng CKY, Kinoshita T, Pandey S, Shimazaki K, Assmann SM. (2004) Abscisic acid induces rapid subnuclear reorganization in guard cells. Plant Physiology 134, 1327-1331. doi:10.1104/pp.103.034728
  126. Doi M, Shigenaga A, Emi T, Kinoshita T, Shimazaki K (2004) A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant. J. Exp. Bot. 55, 517-523. doi:10.1093/jxb/erh044
  127. Kinoshita T, Emi T, Tomonaga M, Sakamoto K, Shigenaga A, Doi M, Shimazaki K. (2003) Blue light- and phosphorylation-dependent binding of a 14-3-3 protein to phototropins in stomtal guard cells of broad bean. Plant Physiology 133, 1453-1463. doi:10.1104/pp.103.029629
  128. Zhao J, Onduka T, Kinoshita J. Honsho M, Kinoshita T, Shimazaki K, Ito A. (2003) Dual subcellular distribution of cytochrome b5 in plant, cauliflower, cells. Journal of Biochemistry 133, 115-121. doi:10.1093/jb/mvg009
  129. Kinoshita T. and Shimazaki K. (2002) Biochemical evidence for the requirement of 14-3-3 protein binding in activation of the guard-cell plasma membrane H+-ATPase by blue light. Plant & Cell Physiology 43,1359-1365. doi:10.1093/pcp/pcf167
  130. Li, J., Kinoshita, T., Pandey, S., Ng, C. K.-Y., Gygi, S.T., Shimazaki, K. and Assmann, A.M. (2002) Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature 418: 793-797. doi:10.1038/nature00936
  131. Kinoshita T., Doi M., Suetsugu N., Kagawa T., Wada M. and Shimazaki K. (2001) phot1 and phot2 mediate blue light regulation of stomatal opening. Nature 414, 656-660. doi:10.1038/414656a
  132. Kinoshita T. and Shimazaki K. (2001) Analysis of the phosphorylation level in guard-cell plasma membrane H+-ATPase in response to fusicoccin. Plant & Cell Physiology 42, 424-432. doi:10.1093/pcp/pce055
  133. Emi T., Kinoshita T. and Shimazaki K. (2001) Specific binding of vf14-3-3a isoform to the plasma membrane H+-ATPase in response to blue light and fusicoccin in guard cells of broad bean. Plant Physiology 125, 1115-1125. doi:10.1104/pp.125.2.1115
  134. Tominaga M., Kinoshita T. and Shimazaki K. (2001) Guard-cell chloroplasts provides ATP required for H+ pumping in the plasma membrane and stomatal opening. Plant & Cell Physiology 42, 795-802. doi:10.1093/pcp/pce101
  135. Kinoshita T. and Shimazaki K. (1999) Blue light activates the plasma membrane H+-ATPase by phosphorylation of the C-terminus in stomatal guard cells. EMBO Journal 18, 5548-5558. doi:10.1093/emboj/18.20.5548
  136. Kinoshita T. and Shimazaki K. (1999) Characterization of cytosolic cyclophilin in guard cells from Vicia faba L. Plant & Cell Physiology 40, 53-59. [Abstract]
  137. Shimazaki K., Goh, C. and Kinoshita T. (1999) Involvement of intracellular Ca2+ in blue light-dependent proton pumping in guard cell protoplasts from Vicia faba. Physiologia Plantarum 105, 554-561. doi:10.1034/j.1399-3054.1999.105322.x
  138. Kinoshita T. and Shimazaki K. (1997) Involvement of calyculin A- and okadaic acid-sensitive protein phosphatase in the blue light response of stomatal guard cells. Plant & Cell Physiology 38,1281-1285. [Abstract]
  139. Goh C-H., Kinoshita T., Oku T. and Shimazaki K. (1996) Inhibition of blue-light-dependent H+ pumping by abscisic acid in Vicia guard-cell protoplasts. Plant Physiology 111, 433-440. doi:10.1104/pp.111.2.433
  140. Kinoshita T., Nishimura M. and Shimazaki K. (1995) Cytosolic concentration of Ca2+ regulates the plasma membrane H+-ATPase in guard cells of Fava bean. Plant Cell 7, 1333-1342. doi:10.2307/3870106
  141. Kinoshita T. and Shimazaki K. (1995) Evidence for Ca2+-dependent phosphorylation in vitro in guard cells from Vicia faba L. Plant Science 110, 173-180. doi:10.1016/0168-9452(95)04200-E
  142. Shimazaki, K. and Kinoshita, T. (1995) Analysis of the light signaling pathway in stomatal guard cells. Methods in Cell Biology, 49, 501-513. doi: 10.1016/s0091-679x(08)61476-4
  143. Kinoshita T., Shimazaki K. and Nishimura M. (1993) Phosphorylation and dephosphorylation of guard-cell proteins from Vicia faba L. in response to light and dark. Plant Physiology 102, 917-923. [Abstract]
  144. Shimazaki, K., Omasa, K., Kinoshita, T. and Nishimura, M. (1993) Properties of the signal transduction pathway in the blue light response of stomatal guard cells of Vicia faba and Commelina benghalensis. Plant & Cell Physiology 34, 1321-1327. [Abstract]
  145. Masamoto, K. Kinoshita, T. Shimazaki, K. (1993) Light-induced de-epoxidation of violaxanthin in guard cell protoplasts of Vicia faba. Plant & Cell Physiology 34, 935-938. [Abstract]
  146. Shimazaki K., Kinoshita T. and Nishimura M. (1992) Involvement of calmodulin and calmodulin-dependent myosin light chain kinase in blue light-dependent H+ pumping by guard cell protoplasts from Vicia faba L. Plant Physiology 99, 1416-1421. doi:10.1104/pp.99.4.1416